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Where Have All the Frogs Gone? UV Radiation and Amphibian Declines

Adrienne Howse

articlehighlights

Amphibians are declining worldwide for many reasons, including ultraviolet radiation (UVR) which leads to:

  • damage to the animal’s DNA
  • the inability of the DNA to self-repair
  • higher mortality rates
  • slower development of embryos

February 2001

There is too little research being done on amphibian declines.
Many environmental factors appear to have contributed to the decline, in varying degrees.

It is becoming increasingly apparent that amphibian populations are in decline in a wide variety of geographic regions and habitats.10 However, most reports about this have been anecdotal and few experiments have been based on quantitative field observations. Scientists suspect the declines may be due, but are not limited to, factors such as:1,8,10


Ultraviolet radiation may affect this toad at the Baltimore Zoo. Photographer: Brent Moore Creative Commons

  • Ultraviolet radiation
  • Pesticides
  • Fungi
  • Surfactants
  • Parasites
  • Habitat destruction
  • Water pollution
  • Introduction of game fish
  • Global warming
  • Viral infections
  • Grazing by cattle
  • Alien predators

Why are some populations declining while others are stable?

Most scientists agree that amphibian species, including salamanders, newts, and toads are showing signs of decline in various locations around the globe.1,2 However, it has also been noted that not all species are declining. Some are even on the rise. For example:

Some amphibian species are declining while others seem immune.
  • In recent years the Costa Rica golden toad is thought to have become extinct.7

  • The gastric brooding frog of North America has not been seen since the early eighties.7

  • The Wyoming toad, with numbers in the fifties, and the Houston toad, at populations under ten thousand, are on the “most endangered” list.

  • Species such as the Colorado River toad, Yosemite toad, foothill yellow-legged frog, mountain yellow-legged frog, cascades frog, spotted frog, leopard frog, tailed frog, and Western spade-foot toad are all on California’s protected species list.

Ultraviolet radiation (UVR) appears to be a large factor in some declines.
  • The gray treefrog of the Southeastern United States seems undaunted by the declines.11,1

The problem now is to find out what is causing the declines, and what makes some species immune. While it is likely that more than one variable is behind the declines, many single variables are being indicated. Ultraviolet radiation is a prominent factor in some decline situations, so more attention to date has been focused on this agent.9

UVR is affecting development as well as mortality.

How is UV radiation a factor?

Recent evidence shows that ultraviolet radiation, perhaps linked to ozone depletion, causes the formation of cyclobutane pyrimidine dimers, leading to a higher mortality rate and slower development time in amphibian embryos. If such is the case, increasing ultraviolet radiation could be a major cause of population declines in the amphibian world.

A zoologist at the University of Oregon, Andrew Blaustein, has been working with ultraviolet radiation and amphibians for a number of years. In a 1997 study he and colleagues determined that ultraviolet radiation causes amphibian embryonic deformities.3

UVR damages DNA by triggering the formation of certain molecules (dimers) that are toxic.
  • Multiple studies have shown that ultraviolet radiation causes the formation of cyclobutane pyrimidine dimers (CBPDs), which are cytotoxic, and mutagenic photoproducts of DNA.
  • These ultraviolet photoproducts impede gene expression by blocking transcription.4
  • The photoreactivating enzyme, photolyase, is the most important means for some organisms to repair CBPDs. Certain species, such as the pacific treefrog, had mortality rates unrelated to ultraviolet radiation. This fact is believed to be linked to relatively high levels of photolyase activity. Species in which ultraviolet radiation negatively affects their mortality rate, such as the boreal toad and cascade frog, were found to have low levels of photolyase activity.4,5
UVR has increased and so have amphibian deaths.

The apparent sensitivity of some amphibians to current levels of ultraviolet radiation has lead to a hypothesis that increasing ultraviolet radiation has contributed to global amphibian declines.3,4 This hypothesis could explain the puzzling recent extensive declines of species in relatively undisturbed habitats. Declines are occurring in such diverse habitats that finding a single common cause is not likely. Declines attributable solely to ultraviolet radiation are therefore suspect.

Why is it important to understand the causes for the declines?

Herpetologists have paralleled amphibians to the proverbial canary in the coalmine. Amphibians are natural indicators of our planet’s well-being. They alert us of its condition.

Amphibians are early warning signs of environmental damage.
  • Because frogs and toads lead a double life, meaning both terrestrial and aquatic, they are twice as likely as other vertebrates to be effected by their environment.
  • What makes frogs such important bioindicators is their permeable skin, which makes them highly vulnerable to toxins and threats present in the water, on land, and in the atmosphere.
  • Amphibians are among the first organisms to suffer from the effects of global pollution and climate change — providing an early warning of environmental degradation.
Conclusion: Amphibian research is vital to the understanding of environmental problems.

With many populations on the decline in diverse and even pristine habitats, frogs are the medium for a vital message: the earth is in trouble. More research needs to be done and more factors investigated. Perhaps some of the more recent research will reveal some answers. For example, the Department of Biology at Arizona State University has been awarded a grant to fund the study “Host-Pathogen Biology and the Global Decline of Amphibians.” Groups like DAPTF, Declining Amphibian Populations Task Force, are constantly working to produce a reliable “frog census.”6,7

The time for anecdotal evidence is passed; the time has now come for quantifiable field research.

Adrienne Danielle Howse is a senior at the Mississippi School for Mathematics and Science. She has been studying amphibians for four years and this article is based on her research. Adrienne plans to major in Molecular Biology at Mississippi State University and then veterinary medicine, also at MSU.

Where Have All the Frogs Gone? UV Radiation and Amphibian Declines

BioScience Articles

  • » “Ghosts in Our Midst: Coming to Terms with Amphibian Extinctions.”
    A global mass extinction of amphibians is well under way, driven both by habitat loss and by environmental changes. As amphibian communities in Central America are being decimated by chytrid disease, scientists are working to fashion an emergency response. They are also sending out an urgent warning about what the loss of these environmentally sensitive species may portend. Learn more about the decline of the first vertebrates on Earth in the April 2007, BioScience article by Scott Norris. Free to read.
    http://caliber.ucpress.net/doi/full/10.1641/B570403
  • » “Amphibian Population Declines: Evolutionary Considerations.”
    Numerous factors, such as global environmental changes, habitat destruction, introduced species, diseases, and chemical pollution, appear to be contributing to amphibian population declines. Blaustein and Bancroft (BioScience, May 2007) believe that a better understanding of amphibian population declines requires that scientists and policymakers consider the ecological processes associated with the declines in light of specific evolutionary principles. Free to read.
    http://caliber.ucpress.net/doi/full/10.1641/B570517

“Amphibia: Life history and ecology”

A good educational site about all things amphibian.
http://www.ucmp.berkeley.edu/vertebrates/tetrapods/amphiblh.html

Amphibian Specialist Group

“The Amphibian Specialist Group strives to conserve biological diversity by stimulating, developing, and executing practical programs to conserve amphibians and their habitats around the world.”
http://www.amphibians.org/

Living Planet Report

This World Wildlife Federation report (2004) is “a periodic update on the world’s ecosystems and the human pressures on them through the consumption of renewable natural resources.” Amphibian populations are part of the analysis.
http://www.panda.org/about_wwf/where_we_work/projects/index.cfm?uProjectID=9Z0712

AIBS Online Presentation

View the PowerPoint presentation “Amphibian Population Declines and Some Misconceptions about Natural Selection” by Andrew R. Blaustein, presented at the 2005 evolution symposium “Evolution and the Environment.”
http://www.aibs.org/special-symposia/resources/NABT_Blaustein_2005.pdf

Frogwatch USA

Volunteers learn about the life cycles and calls of local frogs, monitor frogs and toads in local wetland areas, and submit frog data via the website.
http://www.nwf.org/frogwatchUSA/

Frogwatch Canada

Join other citizens in monitoring a frog site during the calling season. Frogwatch participants can help scientists discover where and which frogs are calling. A frog identification chart is provided.
http://www.cnf.ca/naturewatch/frogwatch/id.html

North American Amphibian Monitoring Program

Volunteers are assigned a roadside route where a “frog call” survey is conducted several times during the calling season, to catch the early through late breeding species
http://www.pwrc.usgs.gov/frogquiz/

Adopt-a-Watershed: For the classroom

An integrated K-12 curriculum, which uses a local watershed as a living laboratory for students. Studies include tree identification, soil, animals, fish, and ecosystems. Grade 3 includes stream-side amphibian studies. Click on “curriculum.”
http://www.adopt-a-watershed.org/

Classroom Activities

  1. Allen, Thomas B. (1972). “Vanishing wildlife in North America.” National Geographic. 3:5-20.
  2. Baker, J. (1997). “Ultraviolet light and amphibian declines.” DAPTF [On-line] 1-2-01. http://www.open.ac.uk/science/biosci/research/ecology/J_Baker/DAPTF.UV.html
  3. Blaustein, A. R., P. D. Hoffman, D. G. Hokit, J. M. Kiescker, S. C. Walls, & J. B. Hays. (1994). “UV Repair and resistance to solar UV-B in amphibian eggs: A link to population declines?” Proceedings of the National Academy of Sciences, USA. 91:1791-1795.
  4. Blaustein, A. R., J. H. Kiescker, D. P. Chivers, & R. G. Anthony. (1997). “Ambient UV-B radiation causes deformities in amphibian embryos.” Proceedings of the National Academy of Sciences, USA. 94:13735-13737.
  5. Corn, S. (18 March 1998). “UV-B radiation and amphibian declines: Summary on UVB and amphibians.” Not published. 1-2-01.
  6. Unknown. (1998). “Why do amphibian declines matter?” DAPTF [On-line] 1-2-01. http://www.open.ac.uk/science/biosci/research/ecology/J_Baker/DAPTF.Matter.html
  7. Unknown. (1998). “What are our findings?” DAPTF [On-line] 1-1-01.
  8. Doyle, R. (1998). “Amphibians at risk.” Scientific American. 0898:1-2-01.
  9. Grant, K.P., & L. E. Licht. (1995). “Effects of ultraviolet radiation on life-history stages of anurans from Ontario, Canada.” Canadian Journal of Zoology, 73:2292-2301.
  10. Halliday, T. (1998). “New evidence confirms amphibian declines in protected habitat. DAPTF [On-line] 1-2-01.
  11. IUCN. (1999). “Class: Amphibians and Anura.” Red List of Threatened Animals Database. IUCN [On-line] 1-11-01. http://www.redlist.org

author glossary

Cytotoxic - having a toxic effect on cells
Dimer - a molecule produced when two molecules of the same chemical structure bond together, e.g., cyclobutane pyrimidine dimer
DNA repair - a process in which various enzymes together repair mutational damage to DNA
Gene expression - the manifestation of specific genetic traits of an organism
Mutagenic - inducing genetic mutation
Transcription - the formation of a nucleic acid molecule using a template of another kind of molecule, such as an RNA molecule from a DNA template

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